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目的:探讨SOX9基因在判断前列腺癌恶性程度及预测前列腺癌去势后生化复发的作用。方法:通过Oncomine公共数据库查询SOX9基因在前列腺癌中的表达情况;RT-QPCR法检测SOX9基因在前列腺癌细胞株DU145、LNCap、PC3和正常前列腺细胞株RWPE-1中的表达;免疫组织化学法检测106例前列腺癌癌组织与癌旁组织中的SOX9基因表达情况,并结合前列腺癌患者临床病理参数进行分析;建立SD大鼠手术去势模型,检测SOX9基因在去势后大鼠前列腺组织中的表达情况。结果:SOX9基因在前列腺癌组织中呈高表达;SOX9基因在PC3细胞株中的表达水平显著高于RWPE-1细胞株(P=0.004);106例前列腺癌癌组织中62例呈SOX9阳性表达,癌旁组织中26例呈SOX9阳性表达;SOX9基因在前列腺癌组织中的表达水平明显强于癌旁组织,两者比较,差异有统计学意义(P=0.000);SOX9高表达与前列腺癌患者血清PSA水平(P=0.007)、Gleason score(P=0.034)和肿瘤TNM分期(P=0.013)呈正相关,而与年龄(P=0.179)无明显相关性;对照组和模型组分别有2只、9只SD大鼠前列腺组织SOX9染色阳性,两组比较,差异有统计学意义(P=0.005)。结论:SOX9基因的高表达与肿瘤的恶性程度相关,可结合血清PSA水平对前列腺癌疑似患者进行早期诊断并判断前列腺癌的恶性程度,还可用来预测生化复发速度。
Objective: To investigate the role of SOX9 in determining the malignancy of prostate cancer and predicting the biochemical recurrence after prostate cancer ovariectomy. Methods: SOX9 gene expression in prostate cancer was detected by Oncomine public database. SOX9 gene expression in prostate cancer cell lines DU145, LNCap, PC3 and normal prostate cell line RWPE-1 was detected by RT-QPCR. Immunohistochemistry To detect the expression of SOX9 in 106 cases of prostate cancer tissues and paracancerous tissues, and to analyze the clinicopathological parameters of prostate cancer patients. To establish SD rat operation castration model to detect SOX9 gene expression in prostate tissue of ovariectomized rats The expression of the situation. Results: The SOX9 gene was highly expressed in prostate cancer tissue. The SOX9 gene expression in PC3 cell line was significantly higher than that in RWPE-1 cell line (P = 0.004). In 106 cases of prostate cancer, 62 cases were SOX9 positive , And 26 cases of paracancerous tissues showed positive expression of SOX9. The expression level of SOX9 in prostate cancer tissues was significantly higher than that in paracancerous tissues (P = 0.000). SOX9 overexpression was associated with prostate cancer There was a positive correlation between serum PSA level (P = 0.007), Gleason score (P = 0.034) and TNM stage (P = 0.013), but no significant correlation with age (P = 0.179) Only nine SD rats stained positive for SOX9 in prostate tissue. The difference between the two groups was statistically significant (P = 0.005). Conclusion: The high expression of SOX9 is correlated with the degree of malignancy of tumor. It can be used in the early diagnosis of prostate cancer suspected patients with serum PSA level to determine the malignancy of prostate cancer. It can also be used to predict the rate of biochemical relapse.