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顺铂是多种化疗方案的核心药物,在对人卵巢癌细胞和大鼠肝癌细胞的研究中,发现其具有诱导凋亡的作用。本实验从细胞形态、ＤＮＡ裂解片段、细胞周期、ＤＮＡ断裂点末端标记,凋亡相关基因观察顺铂诱导人肝癌细胞凋亡的作用机理。1 材料与方法1.1 细胞培养及药物人肝癌细胞ＳＭ
Cisplatin is the core drug for many chemotherapy regimens. In the study of human ovarian cancer cells and rat hepatoma cells, it was found to have an apoptosis-inducing effect. In this experiment, the mechanism of cisplatin-induced apoptosis of human hepatocellular carcinoma cells was observed from the cell morphology, DNA cleavage fragment, cell cycle, DNA breakpoint end marker, and apoptosis-related genes. 1 Materials and Methods 1.1 Cell Culture and Drug Human Liver Cancer Cells SM