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目的:初步探讨轮泻停药膏敷脐对小鼠肠推进以及对番泻叶所致小鼠腹泻后炎性介质的变化的影响,为临床研究治疗轮状病毒腹泻提供有效依据。方法:将所有实验小鼠随机分为3部分,每部分4组,即空白组、模型组、思密达组及轮泻停药膏组。模型组和空白组给予等量生理盐水,思密达组按(0.06g/mL)0.02mL/g,2次/d灌服,轮泻停药膏组贴敷轮泻停药膏,1剂/d;除空白组,其他组小鼠注射甲硫酸新斯的明,观测小鼠小肠酚红的推进率;观察各组对小鼠给予番泻叶后大便次数增加的变化;检测番泻叶所致小鼠严重腹泻后血清中IL-1β和TNF-α的水平变化。结果:轮泻停药膏组与思密达组比较,小肠的推进距离及推进率差异均有显著性(P<0.05),小鼠大便次数也具有显著性差异(P<0.05);两组的血清IL-1β和TNF-α差异也具有统计学意义(P<0.05)。结论:轮泻停药膏能缓解腹泻程度,保护肠黏膜免受炎性介质的损害,具有潜在的治疗轮状病毒腹泻的疗效。
OBJECTIVE: To investigate the effects of Urethral administration of Urethral withdrawal ointment on the intestinal motility in mice and the changes of inflammatory mediators after diarrhea in mice induced by senna, so as to provide an effective basis for clinical research on treating rotavirus diarrhea. Methods: All the experimental mice were randomly divided into 3 parts, each part 4 groups, namely blank group, model group, Smecta group and diarrhea stopping ointment group. The model group and the blank group were given the same amount of saline. The patients in the Smecta group were fed with 0.02ml / g (0.06g / mL) ; In addition to the blank group, the other groups of mice injected with neostigmine methosulfate to observe the progress of the mouse small intestine phenol red; observed mice in each group after the Senate leaves increased stool frequency; senna caused by detection Serum levels of IL-1β and TNF-α in mice after severe diarrhea. Results: Compared with Smecta treatment group, the propulsion distance and propulsion rate of the small intestine were significantly different (P <0.05) and stool frequency was also significantly different (P <0.05) The difference of serum IL-1β and TNF-α also had statistical significance (P <0.05). Conclusion: The diarrhea stopping ointment can relieve the degree of diarrhea, protect the intestinal mucosa from the damage of inflammatory mediators, and has the potential effect of treating rotavirus diarrhea.