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目的探讨前列腺癌细胞核ＤＮＡ倍体、核仁组成区嗜银蛋白（ＡｇＮＯＲＳ）计数及增殖细胞抗原（ＰＣＮＡ）阳性指数变化与癌分级及预后的关系。②方法应用计算机图像分析技术、银染色方法及免疫组化ＬＳＡＢ法，测定３０例前列腺癌病人ＤＮＡ倍体、ＡｇＮＯＲＳ计数和ＰＣＮＡ阳性指数的变化。③结果前列腺癌ＤＮＡ倍体、ＡｇＮＯＲＳ计数、ＰＣＮＡ阳性指数均明显高于前列腺增生症（ｔ＝７．２６７～７．５０２，Ｐ＜０．０１），并且随着前列腺癌细胞分化程度降低，逐渐增加（Ｈ＝４２．５３４，ｔ＝２．１５６～６．７５６，Ｐ＜０．０５，０．０１；Ｆ＝１９４．７７１，１０５．６０５，ｑ＝１３．２１４～１５．８１３，Ｐ＜０．０１）；２年内死亡者，其ＤＮＡ倍体、ＡｇＮＯＲＳ计数、ＰＣＮＡ阳性指数明显高于存活２年以上者（ｔ＝５．２５６～７．６０８，Ｐ＜０．００１）；前列腺癌病人ＤＮＡ倍体、ＡｇＮＯＲＳ计数、ＰＣＮＡ阳性指数间均存在正相关关系（ｒ＝０．９６０～０．９６６，Ｐ＜０．００１）。④结论前列腺癌ＤＮＡ倍体、ＡｇＮＯＲＳ计数、ＰＣＮＡ阳性指数与癌细胞分化程度有关，可作为前列腺癌的预后指标
Objective To investigate the relationship between DNA ploidy, AgNORS count and PCNA positive index of prostate cancer cells and their clinical significance. Methods The changes of DNA ploidy, AgNORS count and PCNA positive index in 30 patients with prostate cancer were detected by computer image analysis, silver staining and immunohistochemical LSAB method. Results The DNA ploidy, AgNORS count and PCNA positive index of prostate cancer were significantly higher than that of benign prostatic hyperplasia (t = 7.267-7.502, P <0.01), and gradually decreased with the differentiation of prostate cancer cells (H = 42.534, t = 2.156-6.756, P <0.05,0.01; F = 194.771,105.605, q = 13.214-15.813, P < 0.01). The DNA ploidy, AgNORS count and PCNA positive index of those who died within 2 years were significantly higher than those who survived for more than 2 years (t = 5.256-7.608, P <0.001). Prostate cancer patients There was a positive correlation between DNA ploidy, AgNORS count and PCNA positive index (r = 0.960-0.966, P <0.001). ④ Conclusion DNA ploidy of prostate cancer, AgNORS count, PCNA positive index and the degree of differentiation of cancer cells can be used as a prognostic indicator of prostate cancer