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用高效液相色谱法测定人血浆中甲硫哒嗪的浓度。以氯氮平为内标，标本在碱性条件下用叔丁基甲醚（叔丁基甲醚与异丙醇之比为６０：１，ｖ／ｖ）提取，流动相为甲醇：水（７５：２５，内含四甲基乙二胺、冰醋酸分别为０．４５％，０．３５％，ｖ／ｖ），用紫外检测器在２５４ｎｍ进行测定。线性范围为１００～１０００ｎｇ／ｍｌ，最低检出限为５ｎｇ／ｍｌ，绝对回收率为８８．７±４．１％（±ＳＤ）天内与天间变异分别为４．６％和４．１％。本法简单、快速、灵敏、准确，适用于甲硫哒嗪的血药浓度监测及临床药理学研究。
Determination of Thioridazine in Human Plasma by High Performance Liquid Chromatography. Clozapine was used as an internal standard. The samples were extracted with t-butyl methyl ether (tert-butyl methyl ether and isopropanol ratio 60: 1, v / v) under alkaline conditions. The mobile phase consisted of methanol: water Containing tetramethylethylenediamine, glacial acetic acid, respectively, 0.45%, 0.35%, v / v), with a UV detector at 254nm determination. The linear range was 100-1000ng / ml, the minimum detectable limit was 5ng / ml, the absolute recovery was 88.7 ± 4.1% (± SD) The intra-and inter-temporal variations were 4.6% and 4.1% . This method is simple, rapid, sensitive and accurate, suitable for the monitoring of thioridazine plasma concentration and clinical pharmacology.