本文介绍一种抗百日咳杆菌脂多糖(LPS)和丝状血凝素(FHA)单克隆抗体菌落印迹ELISA,检测百日咳杆菌和副百日咳杆菌,取得很好结果.将细菌在包姜氏琼脂平皿上培养48小时,把剪成圆形的硝化纤维素膜放在琼脂表面上,于22℃粘附10分钟.取下该膜,浸泡在15ml三氯甲烷的平皿中10分钟,使细菌溶解.取出膜沥干15分钟,浸泡于含3%明胶的20mM Tris HCl-0.5M缓冲盐水(TBS)(pH7.5)溶液45分钟.将膜与百日咳杆菌 This article describes a colony-blot ELISA of anti-Bordetella lipopolysaccharide (LPS) and filamentous hemagglutinin (FHA) monoclonal antibodies for the detection of Bordetella pertussis and Bordetella pertussis, with good results on a package of ginger agar plates After culturing for 48 hours, the sheared round nitrocellulose membrane was placed on the agar surface and adhered for 10 minutes at 22 ° C. The membrane was removed and soaked in 15 ml of chloroform for 10 minutes to dissolve the bacteria. The membrane was drained for 15 minutes and soaked in a solution of 3% gelatin in 20 mM Tris HCl-0.5 M Buffered Saline (TBS) (pH 7.5) for 45 minutes.The membrane was incubated with Bordetella pertussis