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用人胎肝组织粗提物多克隆抗体对构建的人胎肝cDNA文库进行免疫学筛选,筛选约106重组子后,得到9株阳性克隆。5株阳性克隆核苷酸序列表明:3株为α1珠蛋白cDNA序列,2株为未报道序列。
The constructed human fetal liver cDNA library was immunologically screened with the polyclonal antibody of crude fetal liver tissue extract. After screening about 106 recombinants, 9 positive clones were obtained. Five positive cloned nucleotide sequences showed that: three strains of α1 globin cDNA sequences, two were not reported sequence.