本文较为系统而全面地观察了美洲钩虫体内的生化物质,对了解该虫在宿主体内的生理机能及探讨药物的作用机制有一定意义。 材料与方法 实验用金色仓鼠.每鼠由皮下接种美洲钩虫第3期幼虫500条,感染后55d,杀死阳性动物,从小肠取虫,分别用无水酒精、冷丙酮及10％中性福尔马林固定虫体,制成7～10μm的石蜡连续切片。除显 This article systematically and comprehensively observed the biochemical substances in the hookworm, which is of certain significance to understand the physiological function of the pest in the host and to explore the mechanism of action of the drug. MATERIALS AND METHODS Experimental golden hamsters were inoculated with 500 hamster larvae of the American hookworm subcutaneously. Fifty-five days after the infection, the positive animals were killed and the worms were taken from the small intestine. The animals were treated with anhydrous alcohol, cold acetone and 10% Marlin fixed worms, made of 7 ~ 10μm paraffin serial sections. In addition to significant