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为了探讨喉鳞状细胞癌的生物学行为与血管内皮生长因子C(VEGF-C)和血管内皮生长因子受体3(VEGFR-3)之间的关系,我们采用逆转录聚合酶链式反应技术(RT-PCR),从基因水平上对30例正常喉黏膜组织、喉鳞癌组织及颈淋巴结组织中的VEGF-C、VEGFR-3表达进行检测;并采用蛋白质印迹(western bloting)方法从蛋白水平上对10例正常喉黏膜组织、喉鳞癌组织及颈淋巴结组织中的VEGF-C、VEGFR-3表达进行检测。其结果表现为:对同一患者,VEGF-C、VEGFR-3在喉鳞癌组织、颈淋巴结组织及正常喉黏膜组织中的表达有显著性意义(P<0.05),在喉鳞癌组织中,VEGF-C、VEGFR-3的表达与淋巴结转移以及病理分级有关(P<0.05),而与患者年龄性别、病变部位、T分期无关(P>0.05)。由此我们认为喉鳞状细胞癌生物学行为与VEGF-C和VEGFR-3之间有密切的关系。VEGF-C、VEGFR-3联合检测可望作为判断喉鳞癌患者预后的指标。
To investigate the relationship between the biological behavior of laryngeal squamous cell carcinoma and the expression of vascular endothelial growth factor C (VEGF-C) and vascular endothelial growth factor receptor 3 (VEGFR-3), we used reverse transcription polymerase chain reaction (RT-PCR) was used to detect the expression of VEGF-C and VEGFR-3 in 30 cases of normal laryngeal mucosa, laryngeal squamous cell carcinoma and cervical lymph node from the gene level. Western blotting was used to detect the expression of VEGFR- The expression of VEGF-C and VEGFR-3 in 10 cases of normal laryngeal mucosa, laryngeal squamous cell carcinoma and cervical lymph node was detected. The results showed that the expression of VEGF-C and VEGFR-3 in laryngeal squamous cell carcinoma, cervical lymph node and normal laryngeal mucosa was significantly different in the same patients (P <0.05). In laryngeal squamous cell carcinoma, The expression of VEGF-C and VEGFR-3 was correlated with lymph node metastasis and pathological grade (P <0.05), but not with age, sex, T stage (P> 0.05). Therefore, we believe that the biological behavior of laryngeal squamous cell carcinoma and VEGF-C and VEGFR-3 are closely related. VEGF-C, VEGFR-3 combined detection is expected to be used as a prognostic indicator in patients with laryngeal squamous cell carcinoma.