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AIM:To study the expression of survivin,a novel memberof inhibitors of apoptosis protein(IAP)and its significancein colorectal carcinoma.METHODS:Survivin mRNA expression was evaluated bysemi-quantitative reverse transcriptase polymerase chainreaction(RT-PCR)in 52 colorectal carcinoma samples and48 adjacent normal colorectal tissue samples.PCR productwas sequenced to verify the desired result.Expressions ofsurvivin protein,proliferating cell nuclear antigen labellingindex(PI)and apoptotic index(AI)were detectedimmunohistochemically in 52 human colorectal carcinomas.RESULTS:The expression of survivin mRNA was detectedin a significantly greater proportion of colorectal carcinomasamples than in adjacent normal colorectal tissues(67.3%vs 25%;P<0.01).There was no relationship betweensurvivin mRNA expression in colorectal carcinomas andsex,tumor size,histological types,lymphnode metastasis,distant metastasis and Dukes’stage.The PCR productshared 99% of homology with human counterparts.Survivinexpression was observed immunohistochemically in 27 of52 cases of colorectal carcinoma(51.9%).The AI wassignificently lower in survivin positive group than in survivinnegative group(0.67±0.18% vs 1.14±0.42%;P<0.001),while the PI was greater in survivin positive group than insurvivin negative group(51±22% vs 27±18%,P<0.001).CONCLUSION:Survivin is a spedal tumor marker independentof histopathological characteristics.It may play an importantrole during human colorectal tumorigenesis by inhibitingapoptosis and accelerating proliferative activity of colorectaltumor cells.
AIM: To study the expression of survivin, a novel member of inhibitors of apoptosis protein (IAP) and its significancein colorectal carcinoma. METHODS: Survivin mRNA expression was evaluated bysemi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) in 52 colorectal carcinoma samples and 48 adjacent normal colorectal tissue samples. PCR product was sequenced to verify the desired result. Expressions of survivin protein, proliferating cell nuclear antigen labellingindex (PI) and apoptotic index (AI) were detected immunohistochemically in 52 human colorectal carcinomas. a significant greater proportion of colorectal carcinomas samples than in adjacent normal colorectal tissues (67.3% vs 25%; P <0.01). There was no relationship betweensurvivin mRNA expression in colorectal carcinomas and sex, tumor size, histological types, lymphnode metastasis, distant metastasis and Dukes ’stage. PCR productshared 99% of homology with human counterparts. Survivi The AI wassignificently lower in survivin positive group than in survivinnegative group (0.67 ± 0.18% vs 1.14 ± 0.42%; P <0.001), while the PI was greater in (51.9% survivin positive group than insurvivin negative group (51 ± 22% vs 27 ± 18%, P <0.001) .CONCLUSION: Survivin is a spedal tumor marker independent of histopathological characteristics. It may play an importantrole during human colorectal tumorigenesis by inhibitingapoptosis and accelerating proliferative activity of colorectaltumor cells.