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目的制备尼群地平脂质体,测定脂质体的包封率。方法采用单相溶液冻干法制备尼群地平脂质体,用超滤法分离脂质体和游离药物,紫外分光光度法测定其含药量和包封率,并考察了冻干产物的稳定性。结果尼群地平脂质体的含药量为(0.60±0.02)g·L-1,包封率为(65.1±2.1)%,长期放置,包封率变化不大。结论采用单相溶液冻干法制备尼群地平脂质体和紫外分光光度法测定脂质体包封率,方法可行。
Objective To prepare nitrendipine liposomes and determine the encapsulation efficiency of liposomes. Methods Liposomes were prepared by single-phase lyophilization of nitrendipine liposomes, liposomes and free drugs were separated by ultrafiltration. The drug content and entrapment efficiency were determined by ultraviolet spectrophotometry. The stability of freeze-dried products was also investigated Sex. Results The content of nitrendipine liposome was (0.60 ± 0.02) g · L-1, and the entrapment efficiency was (65.1 ± 2.1)%. Conclusion The preparation method of nitrendipine liposomes and UV spectrophotometry for the determination of liposome entrapment efficiency by single phase lyophilization is feasible.