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目的:探讨救脑宁注射液对谷氨酸(Glu)、5-羟色胺(5-HT)诱发大鼠皮质神经细胞内游离钙(〔Ca2+〕i)超负荷的影响。方法:以Fura-2/AM为细胞内钙离子的荧光指示剂,用双波长荧光分光光度计测定了急性分离的新生大鼠神经细胞〔Ca2+〕i的变化,观察Glu、5-HT对〔Ca2+〕i的影响及救脑宁注射液的干预作用。结果:Glu和5-HT剂量依赖性引起〔Ca2+〕i增加,导致细胞内钙超载;救脑宁注射液可显著降低细胞内钙的升高,保护神经细胞免受Glu和5-HT的损害。结论:救脑宁注射液对中风病的治疗作用与其对抗Glu、5-HT等毒性物质的作用,抑制受体依赖性钙通道的开放,减少外钙的流入,有效地防止钙超载等机制有关。
Objective: To investigate the effect of Jiunaoning injection on intracellular free calcium ([Ca2+]i) overload induced by glutamate (Glu) and 5-hydroxytryptamine (5-HT) in rat cortical neurons. METHODS: Fura-2/AM was used as the fluorescent indicator of intracellular calcium ion, and the changes of [Ca2+]i in acutely isolated neonatal rat neurons were measured using a dual-wavelength spectrofluorometer, and Glu and 5-HT pairs were observed. The effect of Ca2+〕i and the intervention of Jiunaoning Injection. RESULTS: Glu and 5-HT caused dose-dependent increase of [Ca2+]i, leading to intracellular calcium overload; Jiunaoning Injection could significantly reduce the increase of intracellular calcium and protect nerve cells from damage of Glu and 5-HT. . Conclusion: The effect of Jiunaoning Injection on apoplexy and its action against Glu, 5-HT and other toxic substances inhibits the opening of receptor-dependent calcium channels, reduces the influx of extracellular calcium, and effectively prevents mechanisms such as calcium overload. .