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目的研究焦炉工人外周血T淋巴细胞核仁区嗜银蛋白(AgNOR)是否可作为多环芳烃暴露的效应标志。方法选取52名焦化厂职工(按焦炉逸散物暴露水平分为高、中、低暴露组)和10名非职业多环芳烃暴露人员(对照组)作为研究对象。采集外周血,经培养、制片、银染,用核仁银染面积/细胞核面积(I/S)衡量T淋巴细胞AgNOR相对含量;同时检测尿1羟基芘的水平,作为接触多环芳烃的内暴露标志。结果高、中、低暴露组和对照组工人尿1羟基芘的平均浓度分别为(16.56±2.77)、(7.17±3.05)、(3.30±2.77)和(3.04±1.58)μmol/mol肌酐,高暴露组与低暴露组和对照组比较,差异有统计学意义;高、中、低暴露组和对照组工人T淋巴细胞AgNOR的I/S值分别为0.056±0.010、0.065±0.013、0.067±0.008和0.076±0.007,高暴露组与其他3组比较以及中、低暴露组与对照组比较,差异均有统计学意义。结论职业多环芳烃暴露可导致尿1羟基芘水平上升和外周血T淋巴细胞AgNOR相对含量下降,提示多环芳烃暴露可损伤T细胞免疫功能,AgNOR有可能作为多环芳烃暴露的效应标志。
Objective To study whether AgNOR in peripheral blood T lymphocytes of coke oven workers can be used as a marker of the effects of PAH exposure. Methods Fifty-two workers in coking plant (exposed to high, medium and low exposure groups according to the level of coke oven exposure) and 10 non-occupational PAHs (control group) were selected as research subjects. Peripheral blood was harvested and the relative content of AgNOR in T lymphocytes was measured by culture, preparation, silver staining and the nucleolar silver staining area / nucleus area (I / S). At the same time, the level of urinary 1-hydroxypyrene Internal exposure sign. Results The average concentrations of urinary 1-hydroxypyrene were (16.56 ± 2.77), (7.17 ± 3.05), (3.30 ± 2.77) and (3.04 ± 1.58) μmol / mol creatinine in high, middle and low exposed and control groups respectively The I / S values of T lymphocyte AgNOR in exposed group and control group were 0.056 ± 0.010,0.065 ± 0.013 and 0.067 ± 0.008 respectively, compared with low exposure group and control group And 0.076 ± 0.007 respectively. There was significant difference between the high exposure group and the other three groups and between the medium and low exposure group and the control group. CONCLUSION: Occupational PAH exposure can lead to the increase of urinary 1-hydroxypyrene level and the decrease of AgNOR in peripheral blood T lymphocytes. It suggests that PAH exposure can impair the immune function of T cells, and AgNOR may be an effective sign of PAH exposure.