论文部分内容阅读
目的以黄独为试材,研究不同因素对黄独茎尖培养的影响,以期找到适合黄独茎尖分化成苗的培养基和脱毒检测方法。方法采用植物组织培养的方法进行茎尖培养,采用症状学法、指示植物法和RT-PCR法对茎尖脱毒植株进行病毒检测。结果黄独茎尖的最佳消毒方式是先用70%酒精消毒30s,再用0.1%HgCl2消毒12min;在37℃中热处理7d后再切离0.5mm茎尖效果较佳。茎尖再生芽增殖的最佳培养基是MS+KT2mg/L+NAA0.5mg/L;黄独茎尖再生芽的最适生根培养基为1/2MS+NAA0.5mg/L;黄独脱毒苗最好的移栽基质为珍珠岩-蛭石(2:1);RT-PCR法是检测黄独马铃薯Y病毒(PVY)感染的主要方法,通过病毒学检测,其平均脱毒率为86.5%。结论首次建立了黄独茎尖脱毒培养的体系,为黄独脱毒苗的快速繁殖及工厂化生产奠定了技术基础。
Objective To study the effects of different factors on the culture of shoot tips of Huang et Rhizome with the purpose of finding a suitable culture medium and virus-free detection method for the differentiation of shoots into shoots. Methods Tissue culture method was used to culture shoot tips. Symptom method was used to detect the virus in shoot tip detoxification plants by plant method and RT-PCR method. Results The best way to disinfect yellow stem tips was to sterilize them with 70% alcohol for 30s and then disinfect them with 0.1% HgCl2 for 12 minutes. After heat treatment at 37 ℃ for 7 days, the tips of 0.5mm shoots were better. The optimum culture medium for regeneration of shoot shoots was MS + KT2mg / L + NAA0.5mg / L; the optimal rooting medium for shoot regeneration from shoots was1 / 2MS + NAA0.5mg / L; The best transplanting medium for seedlings was perlite-vermiculite (2: 1). RT-PCR was the main method for detecting PVY infection. Virulence tests showed that the average virus removal rate was 86.5 %. Conclusions The system of detoxification of yellow tip-tip apical dendritic cells was established for the first time, which laid the technical foundation for the rapid propagation and industrialized production of yellow-spotted virus-free seedlings.