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通过非共价键修饰方法制备了兼具良好生物相容性和导电性的磷脂1-棕榈酰-2-油酰甘油-3-磷酸钠-石墨烯(POPG-GP)纳米复合物,并利用TEM,FT-IR,UV-vis,zeta电位等对其形貌和结构进行了表征.由于POPG-GP纳米复合材料在水溶液中呈现出较为明显的负电性,因此,可通过静电自组装方法将辣根过氧化物酶(HRP)进一步组装到POPG-GP修饰的玻碳(POPG-GP/GC)电极上,构筑HRP/POPG-GP/GC修饰电极.电化学实验结果表明,POPG-GP纳米复合物能够有效实现HRP与修饰电极之间的直接电子转移.此外,固载在修饰电极表面的HRP对底物H2O2还表现出良好的电催化活性.HRP/POPG-GP/GC修饰电极对H2O2的检测线性范围为3.5~210μmol/L,最低检出限为1.17μmol/L(S/N=3),灵敏度为356.6 mA?cm-2?M-1,Km值为0.45 mmol/L.
The phospholipid 1-palmitoyl-2-oleoyl glycerol-3-phosphate-graphene (POPG-GP) nanocomposite with good biocompatibility and conductivity was prepared by non-covalent modification. TEM, FT-IR, UV-vis and zeta potential, etc. The morphology and structure of POPG-GP nanocomposites are characterized by their strong negative charge in aqueous solution, The HRP / POPG-GP / GC modified electrode was constructed by further assembly of horseradish peroxidase (HRP) onto the POPG-GP modified glassy carbon (POPG-GP / GC) electrode.The electrochemical experiments showed that the POPG- The complex can effectively transfer electron transfer between HRP and the modified electrode.In addition, the HRP immobilized on the surface of the modified electrode shows good electrocatalytic activity on the substrate H2O2.The effect of HRP / POPG-GP / GC modified electrode on H2O2 The detection limit ranged from 3.5 to 210 μmol / L with the minimum detectable limit of 1.17 μmol / L (S / N = 3). The sensitivity was 356.6 mA? Cm-2? M-1 and the Km was 0.45 mmol / L.