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采用套式逆转录ＰＣＲ方法检测２６例丙型肝炎及丙型肝炎病毒携带者的血清，外周血单个核细胞及其末次洗液中的ＨＣＶＲＮＡ。结果２６例血清中正链ＨＣＶＲＮＡ全部阳性；外周血单个核细胞中正链ＨＣＶＲＮＡ２２例阳性、负键ＨＣＶＲＮＡ１５例阳性；末次洗液中正链ＨＣＶＲＮＡ全部阴性。说明ＨＣＶ不仅感染ＰＢＭＣ，而且在其中复制．与丙型肝炎的复发、慢性化及干扰素治疗的疗效不佳有关。
Nested HCV RNA was detected in 26 cases of hepatitis C and HCV carriers, peripheral blood mononuclear cells and their last washings by nested reverse transcription PCR. Results The positive strand of HCVRNA was positive in all the 26 cases. The positive strand of HCVRNA in peripheral blood mononuclear cells was positive in 22 cases and the negative strand of HCVRNA was positive in 15 cases. Positive strand RNA in the last wash was negative. That HCV is not only infected with PBMC, but also replicated in it. And hepatitis C recurrence, chronic and poor efficacy of interferon treatment.