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介绍了在非水介质中酶促反应的最新进展。较深入地讨论了介质性质对酶的催化活性及立体选择性的影响,有机溶剂中必需水含量对酶催化反应的重要性,描述了非缓冲体系中的盐及其它极性添加剂对酶活性的影响,介绍了在非均相的低共熔体系中进行酶促合成肽的研究。
The latest progress of enzymatic reactions in non-aqueous media is presented. The effects of media properties on the catalytic activity and stereoselectivity of the enzyme, the importance of the necessary water content in the organic solvent on the enzymatic reaction, and the effect of the salt and other polar additives in the non-buffered system on the enzyme activity Influence, the study of the enzymatic synthesis of peptides in a heterogeneous eutectic system is described.