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目的:建立天麻合剂中天麻素的含量测定方法。方法:色谱柱为伊利特Hypersil ODS2-C_(18)分析柱(250mm×4.6mm,5μm);填充剂为十八烷基硅烷键合硅胶;乙腈(A)-0.1%磷酸(B)为流动相梯度洗脱,0~13.5min,A为1.9%,14~23min,A为40%,23.01~30min,A为1.9%;流速为1mL/min;检测波长为220nm;柱温25℃。结果:回归方程:y=1 632 208x-7 841.85;r=0.999 7;线性范围:0.116~1.16μg。结论:用上述高效液相色谱条件测定天麻素含量专属性强、准确、可行。
Objective: To establish gastrodin content determination of Tianma mixture. METHODS: The column was Elliot Hypersil ODS2-C 18 analytical column (250 mm × 4.6 mm, 5 μm); the filler was octadecylsilane bonded silica gel; acetonitrile (A) -0.1% phosphoric acid (B) Phase gradient elution, 0 ~ 13.5min, A was 1.9%, 14 ~ 23min, A was 40%, 23.01 ~ 30min, A was 1.9%, the flow rate was 1mL / min, the detection wavelength was 220nm, the column temperature was 25 ℃. Results: Regression equation: y = 1 632 208x-7 841.85; r = 0.999 7; linear range: 0.116-1.16 μg. Conclusion: The HPLC determination of gastrodin content with the specificity, accuracy and feasibility.