文章在单因素试验的基础上,采用L9(33)正交试验对苜蓿叶蛋白提取工艺进行研究。结果表明,最佳工艺条件组合为:料液比1∶20、加盐量4%、pH为5,在此条件下可溶性蛋白的提取率可以达到19.62%。影响可溶性蛋白提取率的因素依次为:料液比>加盐量>pH,絮凝温度在60℃、时间为15min时效果最好。 Based on the single factor experiment, the paper studied the extraction technology of alfalfa leaf protein by L9 (33) orthogonal test. The results showed that the optimum conditions were as follows: the ratio of solid to liquid was 1:20, the amount of salt added was 4%, the pH was 5, the extraction rate of soluble protein could reach 19.62%. The factors influencing the extraction rate of soluble protein were as follows: solid-liquid ratio> salinity> pH, flocculation temperature at 60 ℃, the best time was 15min.