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目的分析胶质瘤U87中的CD133阳性(CD133+)和阴性(CD133-)细胞,建立差异性基因文库。方法通过磁珠选择分离U87细胞中的CD133+和CD133-细胞,利用AgilentHuman 1Aoligo芯片检测2种细胞总体基因,分析结果,建立差异性基因文库。结果基因组学研究中,CD133+和CD133-U87细胞比较,差异性基因数为840个;其中,上调基因759个,下调基因81个。在各基因功能分组中,与翻译调控功能相关的基因,差异性基因所占比率最大,为14.9%;各功能组之间差异性基因比率均有统计学意义(P<0.01)。结论通过基因组学研究,建立了CD133+和CD133-U87细胞差异性基因文库,为进一步研究脑肿瘤干细胞提供了线索。
Objective To analyze CD133 positive (CD133 +) and negative (CD133-) cells in glioma U87 and establish a differential gene library. Methods The CD133 + and CD133- cells in U87 cells were isolated by magnetic beads. The total cell number of the two cells was detected by Agilent Human 1Aoligo chip. The differential gene library was established by analyzing the results. Results In genomic study, the number of differentially expressed genes in CD133 + and CD133-U87 cells was 840, of which 759 were up-regulated and 81 were down-regulated. Among all the functional groups, the percentage of genes related to translation regulation function was the highest, accounting for 14.9%. The percentage of differentially expressed genes in each functional group was statistically significant (P <0.01). Conclusion The genomic library of CD133 + and CD133-U87 cells was established by genomics study, providing clues for further study of brain tumor stem cells.