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目的:探讨新疆哈萨克族食管癌组织ras基因的变化及临床意义。方法:采用RFLP和SSCP法对48例原发性食管癌组织进行Ha-ras,Ki-ras基因突变的检测。结果:48例食管癌中,Ha-ras基因外显子的第12 位密码子点突变有10 例,哈萨克族、汉族和维吾尔族的突变分别为4/22、5/21 和1/5,而未发现Ki-ras基因的突变。结论:Ha-ras 癌基因突变是食管癌组织的一种标志物。实验结果进一步支持在食管癌的发生、发展中有多种基因的改变。
Objective: To investigate the changes of ras gene in Kazakh esophageal cancer tissues and its clinical significance. Methods: The mutations of Ha-ras and Ki-ras in 48 primary esophageal cancer tissues were detected by RFLP and SSCP. Results: In 48 cases of esophageal cancer, there were 10 cases of codon 12 mutation in exon of Ha-ras gene, and 4/22, 5/21 and 1/5 in Kazakh, Han and Uygur respectively, No mutation of Ki-ras gene was found. Conclusion: Ha-ras oncogene mutation is a marker of esophageal cancer. The experimental results further support the occurrence and development of esophageal cancer in a variety of gene changes.