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Objective To investigate the antitumor mechanism of interleukin 2 (IL 2) and interleukin 6 (IL 6) gene therapy Methods Liposome encapsulated IL 2 DNA and IL 6 DNA were intraperitoneally (i p ) injected into mouse lymphoma cell line (EL 4) lymphoma bearing mice Macrophage function (M) from the mice was assessed Results Cytotoxicity, major histocompatibility (MHC) Ⅱ expression and IL 1 and TNF secretion of the macrophages all augmented after i p injection of liposome encapsulated IL 2 DNA or IL 6 DNA More efficient activation of macrophages was observed in mice treated with liposome encapsulated IL 2 DNA than IL 6 DNA IL 2 gene therapy combined with IL 6 gene therapy showed the maximal activation of macrophages in the lymphoma bearing mice Conclusion IL 2 and IL 6 gene therapy can relieve the supression of macrophages of the lymphoma bearing mice, and efficiently activate the antitumor immune responses
Objective To investigate the antitumor mechanism of interleukin 2 (IL 2) and interleukin 6 (IL 6) gene therapy Methods Liposome encapsulated IL 2 DNA and IL 6 DNA were injected intraperitoneally (ip) into mouse lymphoma cell line (EL 4) lymphoma bearing mice Macrophage function (M) from the mice was assessed Results Cytotoxicity, major histocompatibility (MHC) Ⅱ expression and IL 1 and TNF secretion of the macrophages all augmented after ip injection of liposome encapsulated IL 2 DNA or IL 6 DNA More efficient activation of macrophages was observed in mice treated with liposome encapsulated IL 2 DNA than IL 6 DNA IL 2 gene therapy combined with IL 6 gene therapy showed the maximal activation of macrophages in the lymphoma bearing mice Conclusion IL 2 and IL 6 gene therapy can relieve the supression of macrophages of the lymphoma bearing mice, and efficiently activate the antitumor immune responses