论文部分内容阅读
目的 :观察黄芩苷对人胃癌细胞株MGC-803和BGC-823细胞迁移能力的影响,并探讨可能的作用机制。方法 :应用细胞划痕愈合实验和Transwell小室迁移实验观察黄芩苷对MGC-803和BGC-823细胞迁移能力的影响。分别采用实时荧光定量PCR法和蛋白质印迹法检测p53、人第10号染色体缺失的磷酸酶及张力蛋白同源的基因(phosphatase and tensin homolog deleted on chromosome ten,PTEN)、组织金属蛋白酶抑制因子(3tissue inhibitor of metalloproteinase 3,TIMP3)以及基质金属蛋白酶3(matrix metalloproteinase-3,MMP3)mR NA和蛋白的表达水平。结果:黄芩苷能明显抑制MGC-803和BGC-823细胞的迁移能力(P值均<0.01)。实时荧光定量PCR和蛋白质印迹法检测结果显示,黄芩苷可上调MGC-803和BGC-823细胞中p53、PTEN和TIMP3 mRNA以及蛋白的表达水平,而下调MMP3 mRNA和蛋白的表达水平(P值均<0.01)结论 :黄芩苷可抑制人胃癌MGC-803和BGC-823细胞的迁移,其作用机制与上调p53、PTEN和TIMP3的表达及下调MMP3的表达有关。
Objective: To observe the effect of baicalin on the migration of human gastric cancer cell lines MGC-803 and BGC-823 cells and to explore the possible mechanism. Methods: The effects of baicalin on the migration of MGC-803 and BGC-823 cells were observed by cell scratch healing assay and Transwell chamber migration assay. Real-time fluorescence quantitative PCR and Western blotting were used to detect the expression of p53, phosphatase and tensin homolog deleted on chromosome ten (PTEN), tissue inhibitor of metalloproteinase 3 tissue inhibitor of metalloproteinase 3, TIMP3) and matrix metalloproteinase-3 (MMP3) mRNA and protein levels. Results: Baicalin significantly inhibited the migration of MGC-803 and BGC-823 cells (all P <0.01). Real-time quantitative PCR and Western blotting results showed that baicalin up-regulated the mRNA and protein expression of p53, PTEN and TIMP3 in MGC-803 and BGC-823 cells, while decreased the expression of MMP3 mRNA and protein <0.01) .Conclusion: Baicalin can inhibit the migration of human gastric cancer cell line MGC-803 and BGC-823, and its mechanism may be related to up-regulating the expression of p53, PTEN and TIMP3 and down-regulating the expression of MMP3.