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目的 研究全反式视黄酸 (ATRA)对胃癌细胞实验性肝转移能力的影响。方法 将胃癌细胞BGC 82 3和MKN 45注射于裸小鼠脾包膜下 ,隔日给裸小鼠灌服ATRA。术后 6周处死裸小鼠 ,解剖脾脏及肝脏肿瘤并用免疫组织化学方法测定其肿瘤血管密度 ;以放射免疫法测定血清癌胚抗原 (CEA)。结果 ATRA抑制胃癌细胞脾包膜下移植瘤的生长及其肝转移 ,抑制率分别为 5 0 .0 %(BGC 82 3)和 33.3% (MKN 45 ) ;使脾移植瘤和肝转移瘤的肿瘤血管密度降低。ATRA还可使肝转移模型鼠血清CEA含量分别下降 5 0 .2 4% (BCG 82 3)和 48.10 % (MKN 45 )。结论 ATRA能有效抑制胃癌细胞的实验性肝转移 ,从而抑制胃癌细胞分泌CEA以及抑制肿瘤血管的形成。
Objective To study the effect of all-trans retinoic acid (ATRA) on experimental liver metastasis of gastric cancer cells. Methods Gastric cancer cells BGC 82 3 and MKN 45 were injected into the subcapsules of nude mice, and nude mice were given ATRA every other day. Naked mice were sacrificed 6 weeks after operation. Spleen and liver tumors were dissected and their tumor vascular density was measured by immunohistochemistry. Serum carcinoembryonic antigen (CEA) was measured by radioimmunoassay. Results ATRA inhibited the growth and liver metastasis of transplanted tumors subcutaneously in the spleen of gastric cancer cells. The inhibitory rates were 50% (BGC 82 3) and 33.3% (MKN 45 ), respectively; the tumors of the transplanted spleen and liver metastases Blood vessel density decreases. ATRA also reduced serum CEA levels in liver metastases rats by 50.24% (BCG 82 3) and 48.10% (MKN 45), respectively. Conclusion ATRA can effectively inhibit the experimental liver metastasis of gastric cancer cells, thereby inhibiting the secretion of CEA in gastric cancer cells and inhibiting the formation of tumor blood vessels.