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利用实时定量PCR方法,检测大豆P39-1基因在大豆各组织中的表达方式,结果显示该基因在大豆根、茎、叶中的表达活性低,而在花和种子中的相对表达活性极高。利用PCR方法,克隆大豆P39-1基因5’端上游2 000 bp序列,命名为P39-1-p。在线启动子预测分析表明P39-1-p序列中含有多种典型的种子特异表达元件和花特异表达的元件,如SEF1 motif、SEF3motif、SEF4 motif、E-box、G-box、AACACA、AACA、ACGT、CCAA;52-box、ntp303-box、GTGA、TACPyATbox,推测大豆P39-1-p启动子具有调控下游基因大量表达在花和种子中的特性。
The real-time PCR method was used to detect the expression pattern of soybean P39-1 gene in soybean tissues. The results showed that the expression level of P39-1 gene in soybean roots, stems and leaves was low, while the relative expression activity in flowers and seeds was extremely high . The 2 000 bp upstream of 5 ’end of P39-1 gene of soybean was cloned by PCR and named as P39-1-p. The online promoter prediction analysis showed that the P39-1-p sequence contains many typical seed-specific expression elements and flower-specific expression elements such as SEF1 motif, SEF3 motif, SEF4 motif, E-box, G- box, AACACA, ACGT, CCAA, 52-box, ntp303-box, GTGA and TACPyATbox, respectively. It was speculated that the soybean P39-1-p promoter regulates the expression of downstream genes in flowers and seeds.