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目的：通过对一个氨基甙类抗生素致聋家系１１名成员的线粒体ＤＮＡ进行分析，探讨该病的遗传方式及与线粒体ＤＮＡ突变的关系。方法：对１１名成员外周血线粒体ＤＮＡ进行ＰＣＲ－ＲＦＬＰ及测序分析。结果：８例标本的ＰＣＲ－ＲＦＬＰ结果异常，测序表明其均存在１５５５位点Ｃ－Ｔ突变。结论：氨基甙类抗生素致聋与线粒体ＤＮＡ突变有关，该家系呈典型母系遗传。
Objective: To analyze the mitochondrial DNA of 11 members of an aminoglycoside-induced deaf family and discuss the genetic mode of the disease and its relationship with mitochondrial DNA mutations. Methods: The mitochondrial DNA of peripheral blood of 11 members were analyzed by PCR-RFLP and sequencing. Results: The results of PCR-RFLP in 8 specimens were abnormal. Sequencing showed that there were C-T mutations at 1555 sites. Conclusion: Aminoglycoside-induced deafness is related to mitochondrial DNA mutation. The pedigree is typical maternally inherited.