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采用酵母杂合启动子PADH2－CUP1或PADH2－GAPDH及终止子TADH1，构建了一系列酵母表达载体．在这些表达载体中插入乙肝病毒表面抗原S－preS1融合基因SA－28后，将合乙肝病毒表面抗原的表达单元克隆至高稳定质粒PHC11的BamHI位点．然后将表达质粒分别转化酿酒酵母Y16，Y19．对SA－28基因表达的研究表明，在酵母菌胞内实现了SA－28基因的高表达，且表达受葡萄糖浓度调控．
Using yeast hybrid promoter PADH2-CUP1 or PADH2-GAPDH and terminator TADH1, a series of yeast expression vectors were constructed. After inserting the S-preS1 fusion gene SA-28 of the hepatitis B virus surface antigen into these expression vectors, the expression unit of hepatitis B virus surface antigen was cloned into the BamHI site of the highly stable plasmid PHC11. The expression plasmids were then transformed into Saccharomyces cerevisiae Y16, Y19, respectively. Studies on SA-28 gene expression show that SA-28 gene is highly expressed in yeast cells, and the expression is regulated by glucose concentration.