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本文用凝乳法和中国药典1990版牛血红蛋白分光光度法对不同贮存温度和时间内胃蛋白酶活力变化进行了实验观测,从而对各地规范定的凝乳法测定进行评价,现报导如下:1 仪器与药品53W 紫外分光光度仪(上海光学仪器厂);电热恒温水浴锅(江苏常熟医疗器械厂);PHS-3C 型酸度计(上海雷磁仪器厂);胃蛋白酶(1∶1200)(青岛生物化学制药厂,批号900115);L-酪氨酸对照品(608-9003)(中国药品生物制品检定所);牛血红蛋白(608-9104)(中国药品生物制品检定所);氢氧化钠(GR);三氯乙酸,盐酸(AR)。
In this paper, with the curd method and Chinese Pharmacopoeia 1990 edition of bovine hemoglobin spectrophotometry of pepsin activity changes in different storage temperature and time were observed experimentally to regulate the curd around the determination of the evaluation are reported as follows: 1 instrument And 53W UV spectrophotometer (Shanghai Optical Instrument Factory); electric heated water bath (Jiangsu Changshu Medical Devices Factory); PHS-3C-type acid meter (Shanghai Lei Magnetic Instrument Factory); pepsin (1: 1200) Chemical Pharmacy, batch number 900115); L-tyrosine reference substance (608-9003) (China Pharmaceutical and Biological Products Inspection); bovine hemoglobin (608-9104) (China Pharmaceutical and Biological Products Inspection); sodium hydroxide (GR ); Trichloroacetic acid, hydrochloric acid (AR).