论文部分内容阅读
[目的]探讨哺乳动物睾丸决定因子样 HMG盒9(SOX9)基因表达对脑胶质瘤细胞增殖能力的影响.[方法]选取脑胶质瘤 U87 细胞,随机分为对照组、空白转染组和干扰组,其中空白转染组转染慢病毒,干扰组转染 SOX9慢病毒,采用 MTT法检测细胞增殖,DAPI细胞核染色检测细胞凋亡,实时荧光定量 PCR检测 SOX9 mRNA表达,Western blot检测 SOX9、SOX2、Nestin蛋白表达.[结果]干扰组细胞 SOX9 mRNA表达明显低于空白转染组、对照组,空白转染组明显低于对照组(P ＜0.05);干扰组细胞凋亡率明显高于空白转染组、对照组,空白转染组明显高于对照组(P ＜0.05).干扰组培养48 h、72 h时OD值明显低于空白转染组、对照组,空白转染组培养 48 h、72 h时的 OD值明显低于对照组(P ＜0.05).干扰组 SOX9、SOX2、Nestin蛋白相对表达量明显低于空白转染组、对照组,而空白转染组 SOX9、SOX27 蛋白相对表达量明显低于对照组(P ＜0.05).[结论]SOX9 基因表达可抑制脑胶质瘤细胞增殖,促进细胞凋亡,下调 SOX2、Nestin蛋白表达.“,”[Objective]To investigate the effect of testis determinant HMG box 9 (Sox9)gene expression on the proliferation of glioma cells.s.[Methods]The brain glioma U87 cells were selected and randomly divided into control group,blank transfection group and interference group.The blank transfection group was transfected with lentivirus,the interference group was transfected with Sox9 lentivirus.MTT method was used to detect cell proliferation,DAPI nuclear staining was used to detect cell apoptosis,real time fluorescent quanti tative PCR was used to detect Sox9 mRNA expression,Western blot was used to detect Sox9,Sox2,nestin protein expression.[Results]The expression of Sox9 mRNA in the interference group was significantly lower than that in the blank transfection group and the control group,the blank transfection group was significantly lower than that in the control group (P ＜0.05),the apoptosis rate in the interference group was significantly higher than that in the blank transfection group and the control group,and the blank transfection group was significantly higher than that in the control group (P ＜0.05).The OD value of the interference group at 48 h and 72 h was significantly lower than that of the blank transfection group and the control group,and that of the blank transfection group at 48 h and 72 h was significantly lower than that of the control group (P ＜0.05).The relative expression of Sox9,Sox2 and nestin protein in the interference group was significantly lower than that in the blank transfection group and the control group,while the relative expression of Sox9 and sox27 protein in the blank transfection group was significantly lower than that in the control group (P ＜0.05).[Conclusion]Sox9 gene expression can inhibit the proliferation of glioma cells,promote apoptosis and down regulate the expression of Sox2 and nestin protein.