论文部分内容阅读
目的比较r h KG F、CB L B 5 0 2、W R 2 7 2 1对放射性口腔黏膜炎(R O M)的防治作用。方法健康雄性C57BL/6J小鼠随机分为正常对照组、照射对照组、rh KGF组、CBLB502组及WR2721组。其中24只小鼠观察17Gy头颈部照射小鼠的30d存活率及体重变化,另外20只小鼠利用1%甲苯胺蓝染色法观察照射小鼠舌组织溃疡情况,HE染色观察舌组织病理学改变,Ki-67免疫组化检测黏膜角化上皮细胞增殖情况。结果与照射对照组相比,rh KGF和WR2721给药组小鼠30d存活率显著提高,体重恢复较快,角化上皮细胞增殖显著,未发生明显的口腔黏膜炎;CBLB502组小鼠的存活率、体重变化及组织病理学指标与照射对照组比较差异无统计学意义(P>0.05)。结论 rh KGF和WR2721对放射性口腔黏膜炎有较好的防治作用,而CBLB502并不能减轻放射性口腔黏膜炎的发生。
Objective To compare the preventive and therapeutic effects of r K KG F, CB L B 5 0 2 and W R 2 7 2 1 on radiation-induced oral mucositis (R O M). Methods Healthy male C57BL / 6J mice were randomly divided into normal control group, irradiation control group, rh KGF group, CBLB502 group and WR2721 group. Twenty-four mice were used to observe the 30-day survival rate and body weight of 17 Gy head and neck irradiated mice. Twenty mice were used to observe ulceration of tongue tissues by 1% toluidine blue staining. HE staining was used to observe tongue histopathology Change, Ki-67 immunohistochemical detection of mucosal epithelial cell proliferation. Results Compared with the irradiated control group, the survival rate of mice in rhKGF and WR2721 groups increased significantly at 30d, the body weight recovered rapidly, the proliferation of keratinized epithelial cells was significant, and no obvious oral mucositis occurred. The survival rate of mice in CBLB502 group , Body weight changes and histopathological parameters compared with the irradiated control group showed no significant difference (P> 0.05). Conclusion rh KGF and WR2721 have good preventive and therapeutic effects on radiation-induced oral mucositis, while CBLB502 does not reduce the incidence of radiation-induced oral mucositis.