论文部分内容阅读
Objective:To construct a eukaryotic expression plasmid pcDNA3.1(-)-Humanin.Methods:The recombinant plasmidpGEMEX-1-Humanin was digested with restriction endonucleases BamH Ⅰ and Hind Ⅲ and the Humanin gene fragments,about100 bp length,were obtained.Then the Humanin gene fragments were inserted into eukaryotic expression vector pcDNA3.1(-)andthe recombinant plasmids pcDNA3.1(-)-Humanin were identified by sequencing.Results:Recombinant plasmid DNA success-fully produced a band which had the same size as that of the thimauin positive control.The sequence of recombinant plasmidsaccorded with the Humnain gene sequence.Conclusions:A eukaryotic expression plasmid of Humanin was successfully con-structed.
Objective: To construct an eukaryotic expression plasmid pcDNA3.1 (-) - Human in. Methods: The recombinant plasmid pGEMEX-1-Humanin was digested with restriction endonucleases BamH I and Hind III and the Human in gene fragments, about 100 bp length, were obtained. the Humanin gene fragments were inserted into eukaryotic expression vector pcDNA3.1 (-) and the recombinant plasmids pcDNA3.1 (-) - Humanin were identified by sequencing. Results: Recombinant plasmid DNA success-fully produced a band which had the same size as that of the thimauin positive control. The sequence of recombinant plasmidsaccorded with the Humnain gene sequence. Conclusions: A eukaryotic expression plasmid of Humanin was successfully con-structed.