在培养的大鼠垂体前叶细胞悬液内加入内皮素100 nmol/L,可诱发胞浆钙浓度升高,即刻出现一高峰波,随后为低平台波,此双相波约维持1.5 min。高峰波在10 s内出现,若消除细胞外Ca~(2+),不影响高峰波的产生,说明峰波与细胞内储存的Ca~(2+)有关。若加双氢吡啶钙通道拮抗剂硝苯啶(nifedipine)1 μmol/L, Adding endothelin (100 nmol / L) to the cultured rat anterior pituitary cell suspension induced an increase in cytoplasmic calcium concentration, a peak wave appeared immediately followed by a low plateau wave. This biphasic wave was maintained for 1.5 min. The peak wave appeared within 10 s, if the elimination of extracellular Ca ~ (2+), does not affect the peak wave generation, indicating that the peak wave and intracellular storage of Ca ~ (2). If plus calcium dihydropyridine channel antagonist nifedipine (1 μmol / L,