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目的为羌活和独活药材的鉴别提供科学依据。方法采用气相色谱(GC)-氢火焰离子化检测法。色谱柱:DB-1毛细管柱(30 m×0.25 mm×0.25μm);程序升温:起始柱温50℃,8℃.min-1升至85℃保持12 min,4℃.min-1升至155℃保持18 min,12℃.min-1升至250℃保持5 min;气化室温度:250℃;载气:氮气;流速:1.2 mL.min-1;进样量:1.0μL;分流比:10∶1;检测器温度:270℃。结果建立了羌活和独活药材的GC指纹图谱;对不同产地药材分别进行了相似度计算;指纹图谱和相似度计算结果显示,羌活和独活药材挥发油含量具有明显区别。结论本方法可用于羌活和独活药材的定性鉴别。
Objective To provide a scientific basis for the identification of Notopterygii and independent living herbs. Methods Gas chromatography (GC) - hydrogen flame ionization detection method. Column: DB-1 capillary column (30 m × 0.25 mm × 0.25 μm); program temperature: the initial column temperature 50 ℃, 8 ℃ .min-1 rose to 85 ℃ for 12 min, 4 ℃ .min- To 155 ℃ for 18 min, 12 ℃ .min-1 to 250 ℃ for 5 min; gasification chamber temperature: 250 ℃; carrier gas: nitrogen; flow rate: 1.2 mL.min- Split ratio: 10: 1; Detector temperature: 270 ° C. Results The GC fingerprints of Rhizoma et Radix Notopterygii and Radix Angelicae Pubescentis were established. Similarity calculation was carried out on the medicinal materials of different origins. The fingerprint and similarity calculation results showed that the contents of volatile oil in Rhizoma et Radix Notopterygii and Radix Angelicae Pubescentis were significantly different. Conclusion This method can be used for qualitative identification of Notopterygii and independent living herbs.