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目的研究健脾化湿方对脾虚痰湿型肥胖2型糖尿病(T2DM)胰岛素抵抗大鼠糖脂代谢的影响,并探讨其机制。方法建立脾虚痰湿型肥胖T2DM胰岛素抵抗大鼠模型,将大鼠分为正常对照组、模型对照组、脾虚痰湿型肥胖糖尿病组、健脾化湿方低、中、高剂量组和二甲双胍组。测量体重及内脏脂肪,检测治疗后空腹血糖(FBG)、空腹胰岛素(FINS)、胰岛素抵抗指数(HOMA-IR)、胰岛素敏感指数(ISI)、血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、游离脂肪酸(FFA)。Western Blot检测肝脏组织PPAR-α蛋白表达情况。结果健脾化湿方可明显降低脾虚痰湿型肥胖T2DM胰岛素抵抗大鼠体重、内脏脂肪、FBG、FINS、HOMA-IR及血清TC、TG、LDL-C、FFA水平,升高ISI,上调肝脏组织PPAR-α蛋白表达,且以高剂量健脾化湿方组效果最佳。结论健脾化湿方可以改善脾虚痰湿型肥胖T2DM胰岛素抵抗大鼠糖脂代谢,其机制可能是通过上调肝脏组织PPAR-α蛋白表达发挥调脂作用。
Objective To study the effect of Jianpi Huashi Decoction on the glucose and lipid metabolism in rats with type 2 diabetes mellitus (T2DM) with spleen deficiency phlegm and its mechanism. Methods To establish the rat model of T2DM with T2DM due to phlegm-dampness of spleen deficiency type, the rats were divided into normal control group, model control group, phlegm and phlegm-dampness obesity diabetes group, low-, medium- and high-dose Jianpi Huashi formula group and metformin group . Body weight and visceral fat were measured. Fasting blood glucose (FBG), fasting insulin (FINS), insulin resistance index (HOMA-IR), insulin sensitivity index (ISI), serum total cholesterol (TC), triglyceride (TG) , High-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), free fatty acid (FFA). Western Blot detection of liver tissue PPAR-α protein expression. Results Jianpi Huashi Decoction could significantly reduce the levels of body weight, visceral fat, FBG, FINS, HOMA-IR and TC, TG, LDL-C and FFA, increase ISI and up-regulate the liver in rats with spleen- PPAR-α protein expression, and high-dose Jianpi Huashi Fang best. Conclusion Jianpi Huashi Decoction can improve glucose and lipid metabolism in rats with insulin resistance of T2DM with phlegm-dampness and phlegm-dampness syndrome, and its mechanism may be that it can regulate lipid metabolism by up-regulating the expression of PPAR-α in liver tissue.