冰冻制片的最大优点是快速,但由于快速固定、快速制片,常因制片质量问题造成诊断误差。冰冻切片固定很主要,若固定不良,细胞肿大、核变形、破裂,可造成假阳性或假阴性。过去我们应用的AAF和Cardon这两种固定液,常有细胞肿大现象和核着色不良现象,为解决此问题,我们从1991年9月开始经反复试验,发现用无水乙醇做冰冻切片 The biggest advantage of frozen tablets is fast, but due to rapid fixation, rapid production, often due to the production quality problems caused by diagnostic errors. Frozen section fixation is very important, if poorly fixed, cell swelling, nuclear deformation, rupture, can cause false positives or false negatives. In the past we used the AAF and Cardon these two fixatives, often the phenomenon of cell swelling and nuclear staining bad phenomenon, in order to solve this problem, we began in September 1991 after repeated tests and found that with anhydrous ethanol to do frozen sections