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听小骨体积小,多采用有机玻璃单体包埋听小骨,我们在此基础上,将听小骨染色后再包埋,使标本更为美观,易于区别与示教,也提高了学生的学习兴趣.具体制作方法如下:1 听小骨的准备将固定过的听小骨经梯度脱水,风干备用.2 染料的选定及配制标本染色多采用油画颜料,但此方法并不适用于听小骨的染色,因油画颜料相对比较粘稠,失真较严重,并且在有机玻璃单体中会皱缩,影响标本外观.若采用组织胚胎学的染色方法,虽然效果满意,但制作过程繁琐,染色材料配制麻烦.我们经过试验以后,发现用普通墨水染色效果较好,基本上不失真,且操作简便.因听小骨体积小,故染色以浅色为宜,以免染色过深造成标本缩小的错觉.我们的经验是少用蓝黑及黑色墨水,选用纯蓝及曙红墨水.从纯蓝及曙红墨水中各取等量墨水混合,稍为摇晃一下,即可得紫红色墨水,此时紫红色墨水对染色来说还相对浓一些,可加入适量蒸馏水充分混合,用毛笔醮墨水在白纸上试到颜色满意为止.纯蓝及曙红墨水也可加入适量蒸馏水稀释.但3种颜色又需保持一定的对比效果,紫红墨水若过度稀释,则会变成近似粉红色,与曙红对比
Listen to the small size of the small bone, the use of plexiglass monomer embedded in the auditory ossicles, on this basis, we will listen to the bony staining and then embedding, the specimens more beautiful, easy to distinguish and teach, but also improve the students’ interest in learning The specific production methods are as follows: 1 Listen to the preparation of small bones will be fixed by the small bone gradient dehydration, air-dried reserve .2 Dye selection and preparation Specimens to dye more use of oil paints, but this method is not suitable for small bony staining, Due to relatively viscous oil paint, the distortion is more serious, and in the plexiglass monomer will shrink, affecting the appearance of the specimen.If the method of tissue embryo staining, although the results are satisfactory, but the production process is cumbersome, preparation of dyeing materials. After we tested, we found that ordinary ink stain better, basically no distortion, and easy to operate.Because the small size of the small bone, it is appropriate to light color, so as not to cause too much staining the specimen narrowed the illusion of our experience Less with blue black and black ink, use pure blue and eosin ink from pure blue and eosin ink each take the same amount of ink mixed, a little shake, you can get magenta ink, purple ink at this time Color is also relatively concentrated, can be added to the right amount of distilled water to fully mix, brush ink on white paper to try to color satisfaction so far. Pure blue and eosin ink can also be added to the amount of distilled water dilution, but the three colors need to maintain a certain Contrast effect, purple ink if excessive dilution, it will become almost pink, compared with the eosin