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目的:探讨丹芍汤对大鼠肾性贫血的治疗作用。方法:随机选取80只健康雄性SD大鼠,10只作正常对照,70只用含0.75%的腺嘌呤饲料喂养,制作肾性贫血模型。模型组随机分为丹芍汤治疗组和生理盐水治疗组。造模6周和丹芍汤治疗4周后,分别测定血清尿素氮(BUN)、肌酐(Cr)和外周血血红蛋白(Hb)、红细胞(RBC)水平,显微镜下观察肾组织的形态学变化。结果①造模6周后,外周血Hb和RBC水平均显著低于正常对照组(95.1±8.5vs161.2±10.3;5.2±0.5vs7.8±0.4,P<0.05);丹芍汤治疗4周后,其水平均显著高于生理盐水治疗组(158.6±14.6vs101.3±8.6;7.9±0.4vs5.1±0.3,P<0.05)。②造模6周后,血清BUN和Cr水平均显著高于正常对照组(240.5±10.2vs4.2±0.3;435.0±16.2vs56.1±6.8,P<0.05);丹芍汤治疗4周后,其水平均显著低于生理盐水治疗组(6.2±0.4vs232.1±12.6;68.8±6.2vs423.2±18.0,P<0.05)。③丹芍汤治疗4周后,模型组大鼠肾组织形态结构恢复正常。结论:丹芍汤对大鼠肾性贫血有良好的治疗作用。
Objective: To investigate the therapeutic effect of Dan Shao Tang on renal anemia in rats. Methods: Eighty healthy male Sprague-Dawley rats were randomly selected. Ten of them were normal controls, and 70 were fed with adenine feed containing 0.75% to produce renal anemia model. The model group was randomly divided into Dan Shao Tang treatment group and saline treatment group. After 6 weeks of treatment and Dan Shao Decoction for 4 weeks, the levels of serum urea nitrogen (BUN), creatinine (Cr) and peripheral blood hemoglobin (Hb) and erythrocytes (RBC) were measured and the morphological changes of kidney were observed under microscope. Results ① After 6 weeks of modeling, the levels of Hb and RBC in peripheral blood were significantly lower than those in the normal control group (95.1 ± 8.5 vs 161.2 ± 10.3; 5.2 ± 0.5 vs 7.8 ± 0.4, P <0.05) Weeks, the levels were significantly higher than the saline treatment group (158.6 ± 14.6vs101.3 ± 8.6; 7.9 ± 0.4vs5.1 ± 0.3, P <0.05). ② After 6 weeks of modeling, serum BUN and Cr levels were significantly higher than those in the normal control group (240.5 ± 10.2 vs 4.2 ± 0.3; 435.0 ± 16.2 vs 56.1 ± 6.8, P <0.05) , The levels were significantly lower than the saline treatment group (6.2 ± 0.4vs232.1 ± 12.6; 68.8 ± 6.2vs423.2 ± 18.0, P <0.05). ③ Dan Shao Tang 4 weeks after treatment, the model group rats kidney morphology returned to normal. Conclusion: Dan Shao Tang has a good therapeutic effect on renal anemia in rats.