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目的 :研究L -精氨酸对肾性高血压心肌肥厚大鼠血浆内皮素及左室肌原癌基因c -fosmRNA表达的影响。方法 :采用原位杂交方法检测原癌基因c-fosmRNA的表达 ,并用图像分析仪作半定量分析。用放射免疫法测定血浆内皮素含量。结果 :经过 8周治疗后 ,L -Arg使高血压心肌肥厚大鼠左室肌原癌基因c -fosmRNA表达显著减少 (P <0 .0 1) ,并显著降低血浆内皮素含量 (P <0 0 1)。结论 :L -Arg能降低高血压心肌肥厚大鼠血浆内皮素含量并使左室肌原癌基因c-fosmRNA的表达量减少。
Objective: To investigate the effect of L - arginine on the plasma endothelin and c - fos mRNA expression in left ventricular myocytes of hypertrophic cardiomyopathy rats. Methods: The expression of proto-oncogene c-fos mRNA was detected by in situ hybridization and semi-quantitative analysis was performed with image analyzer. Plasma endothelin levels were determined by radioimmunoassay. Results: After 8 weeks of treatment, L-Arg significantly reduced the expression of c-fos mRNA in left ventricular myocytes in hypertensive rats (P <0.01), and significantly decreased the plasma levels of endothelin (P <0. 01) 0 1). CONCLUSION: L-Arg can decrease plasma endothelin level and decrease the expression of c-fos mRNA in left ventricular myocytes in hypertensive rats with hypertrophic myocardium.