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Objective Constructing replication defect type virus vector of yeast cytosine deaminase gene expression guided by hTERT core promoter modified by OSE2 tandem replicator.Methods Multimer of OSE2 was designed and cloned into the upstream site of hTERT core promoter,then confirmed by sequencing.Shuttle plasmids were constructed using modified hTERT promoter and Fcy1 and cotransfected HEK 293 cells.Recombination yields replication defect type adenovirus.Result HEK 293 cells cotransfected by shuttle plasmid and assisted plasmid appeared typical cytopathic effect 7～10 days later.122bp objective fragment was confirmed by PCR evaluation.Conclusion We successfully constructed the replication defective adenovirus of Fcy1 gene expression guided by hTERT core promoter modified by 3 or 6 copys OSE2.
Objective Constructing replication defect type virus vector of yeast cytosine deaminase gene expression by hTERT core promoter modified by OSE2 tandem replicator. Methods Multimer of OSE2 was designed and cloned into the upstream site of hTERT core promoter, then confirmed by sequencing. Hisut plasmids were constructed using modified hTERT promoter and Fcy1 and cotransfected HEK 293 cells. Recombination yield replication defect type adenovirus. Resulting HEK 293 cells cotransfected by shuttle plasmid and assisted plasmid 7 days to 10 days later.122 bp objective fragment was confirmed by PCR evaluation. Confluence We successfully constructed the replication defective adenovirus of Fcy1 gene expression guided by hTERT core promoter modified by 3 or 6 copys OSE2.