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目的探讨TLR4蛋白在膀胱尿路上皮癌(BUEC)中的表达及意义。方法膀胱BUEC组62例标本经病理复检,WHO和ISUP病理分级具低度恶性潜能的乳头状尿路上皮肿瘤(G1)5例,低度恶性乳头状尿路上皮肿瘤(G2)24例,高度恶性乳头状尿路上皮肿瘤(G3)33例。11例正常膀胱粘膜和13例癌旁膀胱粘膜组织标本作为对照组。应用免疫组化非生物素即用型二步法检测组织TLR4的表达。结果两对照组TLR4均100%呈强阳性表达,G1中60%强阳性、40%弱阳性,G2中21%弱阳性、79%阴性,G3中3%弱阳性、97%阴性,随肿瘤病理分级上升,TLR4阳性表达率逐渐下降。结论TLR4表达与BUEC分级相关,可成为估计肿瘤预后的一项指标。
Objective To investigate the expression of TLR4 protein in bladder urothelial carcinoma (BUEC) and its significance. Methods Sixty-two specimens from the bladder of BUEC group were pathologically reviewed. Five patients with low malignant potential papillary urothelial carcinoma (G1) and 24 patients with low-grade papillary urothelial carcinoma (G2) were screened by WHO and ISUP. Thirty - three cases of highly malignant papillary epithelial tumor (G3). Eleven cases of normal bladder mucosa and 13 cases of adjacent non-cancerous bladder mucosa were used as the control group. Tissue TLR4 expression was detected by immunohistochemical non-biotin-ready two-step method. Results The TLR4 expression in both control groups was strongly positive at 100%, with 60% strongly positive in G1, 40% weak positive in G2, 21% weakly positive in G2, 79% negative, 3% weakly positive in G3, and 97% negative with tumor pathology Grading increased, TLR4 positive expression rate gradually decreased. Conclusion The expression of TLR4 is correlated with the grade of BUEC, which may be an index to evaluate the prognosis of tumor.