论文部分内容阅读
目的探讨急性心肌梗塞发病中免疫指标的变化。方法应用单克隆与多克隆双抗体夹心法检测６６例急性心肌梗塞（ＡＭＩ）病人发病后第１周内血清可溶性白细胞介素－２受体（ｓＩＬ－２Ｒ）的变化，同时检测外周血淋巴细胞膜白细胞介素－２受体（ｍＩＬ－２Ｒ）表达及Ｔ淋巴细胞亚群的变化。结果ＡＭＩ病人血清ｓＩＬ－２Ｒ明显增高，ｍＩＬ－２Ｒ表达明显降低，与对照组比较差异有极显著性意义（Ｐ＜０．００１）。发病后第１ｄ至第７ｄ，ｓＩＬ－２Ｒ增高和ｍＩＬ－２Ｒ表达降低一直比较明显。ＡＭＩ病人ＣＤ４、ＣＤ８和ＣＤ４／ＣＤ８明显低于对照组（Ｐ＜０．００１），以第１ｄ降低最明显，以后逐渐升高，ＣＤ８于第７ｄ恢复正常。４ｄ内死亡者和伴有室性心律失常（ＶＡ）者上述指标变化更明显。结论血清ｓＩＬ－２Ｒ水平、ｍＩＬ－２Ｒ表达和Ｔ淋巴细胞亚群变化与ＡＭＩ病情和预后有密切关系。
Objective To investigate the changes of immune indexes in the pathogenesis of acute myocardial infarction. Methods Serum soluble interleukin-2 receptor (sIL-2R) levels were measured in 66 patients with acute myocardial infarction (AMI) by monoclonal and polyclonal antibody sandwich method. The peripheral blood lymphocyte membrane Interleukin-2 Receptor (mIL-2R) Expression and T Lymphocyte Subsets Changes. Results The serum level of sIL-2R and the expression of mIL-2R in AMI patients were significantly lower than those in control group (P <0.001). From the first day to the seventh day after the onset of disease, the increase of sIL-2R and the decrease of mIL-2R expression were always obvious. The levels of CD4, CD8 and CD4 / CD8 in patients with AMI were significantly lower than those in the control group (P <0.001). The most significant decrease was seen on the first day and then gradually increased. The CD8 returned to normal on the 7th day. 4d death and those with ventricular arrhythmia (VA) above indicators more obvious changes. Conclusion Serum levels of sIL-2R, mIL-2R and T lymphocyte subsets are closely related to the severity and prognosis of AMI.