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1 前言为了鉴定植物品种和测定其遗传纯度,世界上许多种子科学家纷纷将生化和分子标记快速检测技术引入植物育种、种子生产和种子质量检测中应用。目前主要研究和应用的方法有蛋白质和同工酶标记电泳,以及DNA分子标记的RFLP(Restriction Fragment Length Polymorphisru,限制性片段长度多态性)、RAPD(Randcmly Amplifed Polymorphie DNA,随机扩增多态)、PCR(Polymerase Chain Reaction,聚合酶链反应)和AFLP(Amplifed Fragment Length Polymorphism,扩增片段长
1 INTRODUCTION In order to identify plant species and determine their genetic purity, many seed scientists around the world have introduced biochemical and molecular rapid detection techniques into plant breeding, seed production and seed quality testing. At present, the main research and application methods are protein and isoenzyme electrophoresis, as well as the restriction fragment length polymorphism (RFLP), RAPD (Random Amplification Polymorphism DNA) , PCR (Polymerase Chain Reaction) and AFLP (Amplifed Fragment Length Polymorphism)