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肽类(包括降钙素基因相关肽,CGRP)对冠状动脉血管张力的影响已引起人们注意,作者报道了30例胸痛发作后4小时内收入监护病房的急性心肌梗塞(AMI)患者血浆CGRP浓度的变化,该组患者年龄30～83(平均60)岁,其中20例为男性。 16例患者接受静脉链激酶治疗,10例接受静脉尿激酶治疗,其余4例未接受溶解纤维蛋白治疗。患者入院后即刻,30min,1,2,3,6及24小时各采血一次,离心,血浆CGRP浓度用放射免疫法测定。按试剂盒规定,分离出2ml以上血浆,冻干处理,-72摄氏度贮存。测定时在缓冲液中融冻后,用抗人α-CGRP抗血清测定CGRP浓度,这种抗血清与目前能测定的任何其他神经肽不发生交
The impact of peptides (including calcitonin gene-related peptide, CGRP) on coronary vascular tone has drawn attention and the authors reported the plasma CGRP concentrations in 30 patients with acute myocardial infarction (AMI) admitted to the ICU within 4 hours of the onset of chest pain The group of patients aged 30 to 83 (average 60) years of age, of which 20 were male. 16 patients received intravenous streptokinase therapy, 10 patients received intravenous urokinase treatment, and the remaining 4 patients did not receive dissolved fibrin treatment. Patients immediately after admission, 30min, 1, 2, 3, 6 and 24 hours of blood collection, centrifugation, plasma CGRP concentration by radioimmunoassay. According to the provisions of the kit, more than 2ml of plasma was isolated, lyophilized, stored at -72 degrees Celsius. After thawing in the buffer when assayed, the concentration of CGRP was determined with anti-human α-CGRP antiserum, which did not cross with any other neuropeptides that could be assayed at present