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应用多聚酶链延伸反应──限制性片段长度多态性分析法（ＰＣＲ－ＲＦＬＰ）对４３例福尔马林液固定、石蜡包埋肝癌组织癌基因ｒａｓ和抑癌基因Ｐ（５３）点突变进行分析。结果表明，ｒａｓ基因总突变率为４１．９％（１９／４３），其中Ｎ－ｒａｓ第１２位密码子突变率为３７．２％（１６／４３），Ｃ－Ｋｉ－ｒａｓ第１２位密码子突变率为６．９％（３／４３），Ｃ－Ｋｉ－ｒａｓ第１３位、Ｃ－Ｈａ－ｒａｓ第１２和６１位密码子未发现有突变者。Ｐ~（５３）基因第２４９密码子突变率为２０．９％（９／４３），第２４８密码子未发现有突变者。结果提示Ｎ－ｒａｓ第１２密码子和Ｐ（５３）第２４９密码子突变与肝癌的发生和发展有关。
Application of polymerase chain extension reaction-restriction fragment length polymorphism analysis (PCR-RFLP) to carry out 43 point formalin-fixed, paraffin-embedded liver cancer tissue oncogene ras and tumor suppressor gene P (53) point mutations analysis. The results showed that the total mutation rate of ras gene was 41.9% (19/43). The mutation rate of the 12th codon of N-ras was 37.2% (16/43). The C-Ki-ras 12th password was The sub-mutation rate was 6.9% (3/43). No mutations were found in C-Ki-ras No. 13 and C-Ha-ras No. 12 and 61 codons. The mutation rate of the 249 codon of the P53 gene was 20.9% (9/43). No mutation was found at the 248th codon. The results suggest that mutations in N-ras codon 12 and P(53) codon 249 are related to the occurrence and development of liver cancer.