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为了探寻有效的控制副溶血弧菌的方法,从水产品市场的污水中分离出来一株烈性噬菌体qdvp001。采用双层平板法分离纯化噬菌体qdvp001,电镜观察其形态特征,并利用双层平板法测定其生理特性,包括热稳定性试验、最适pH、最佳感染复数及一步生长曲线,然后提取基因组进行酶切和序列分析。结果显示该烈性噬菌体qdvp001头部直径大约为79 nm,尾长大约118 nm,属于肌尾噬菌体科。它对60°C以下的温度耐受力较强,最适pH为7.0-8.0左右,最佳感染复数是0.000 1,感染宿主菌的潜伏期约20 min,裂解期约70 min,并获得部分DNA片段的序列。将获得的DNA序列在NCBI上进行比对,结果显示,qdvp001与其他噬菌体的同源性较低。该噬菌体很可能是一种新发现的噬菌体。
In order to find an effective method for controlling Vibrio parahaemolyticus, a strong bacteriophage qdvp001 was isolated from the sewage of aquatic products market. The phage qdvp001 was isolated and purified by the double-layer plate method. The morphological characteristics of the phage were observed by electron microscope. The physiological characteristics of the phage qdvp001, including the thermostability test, the optimal pH, the optimal multiplicity of infection and the one-step growth curve, Digestion and sequence analysis. The results showed that the potent phage qdvp001 head diameter of about 79 nm, the tail length of about 118 nm, belong to myogenic phage. It is more tolerant to temperatures below 60 ° C, with an optimum pH of about 7.0-8.0, an optimal multiplicity of infection of 0.000 1, an incubation period of about 20 min for the host strain of infection, and about 70 min for the lysis period and a partial DNA Sequence of fragments. The obtained DNA sequence was aligned on NCBI, and the result showed that qdvp001 has low homology with other phage. The phage is likely to be a newly discovered phage.