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作者用1种亲脂荧光素PKH-2标记产单核细胞李氏菌和鼠伤寒沙门氏菌,并用流式细胞仪研究J774A.1.细胞株吞噬作用和兼性胞内菌在细胞内的短期存活情况。标记对细菌的影响研究证明,标记和未标记细菌的生活力、生长动力学和在巨噬细胞内的存活完全相同,表明用PKH-2没有毒性作用。标记细菌培养成活率达70%～90%,细菌的生长(cfu/ml)和平均荧光强度呈反相相关。在迟滞期,标记的产单核细胞性李氏菌荧光强度保持不变,而分裂后每个细胞荧光强度降低50%,证实标记是稳定的。用PKH-2标记的细菌在体外感染吞噬细胞,以标准稀释法或细胞分拣法发现,2种
The authors used a lipophilic fluorescein PKH-2 labeled Listeria monocytogenes and Salmonella typhimurium, and the use of flow cytometry J774A.1 cell line phagocytosis and facultative intracellular bacteria in the short-term survival Happening. Effects of labeling on bacteria Studies have shown that the viability, growth kinetics and viability of macrophages of labeled and unlabeled bacteria are identical, indicating that there is no toxic effect with PKH-2. The bacterial growth rate of labeling was 70% ~ 90%. The growth of bacteria (cfu / ml) and the average fluorescence intensity were inversely correlated. During lag phase, the fluorescence intensity of the labeled Listeria monocytogenes remained unchanged while the fluorescence intensity of each cell was reduced by 50% after division, confirming that the marker was stable. Phagocytes were infected in vitro with PKH-2-labeled bacteria and found by standard dilution or cell sorting, two