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D-双聚体和E片段均为交联型纤维蛋白的降解产物。根据它们的等电点的不同,建立了等电聚焦法纯化D-双聚体和E片段的方法。纯化的D-双聚体分子量约3.32×10~(-22)kg,含γ-γ双聚体片段(分子量1.36×10~(-22)kg)、β链片段(分子量7.30×10~(-23)kg)和α链片段(分子量2.49×10~(-23)kg)。Western Blot结果表明它可被抗D-双聚体单克隆抗体(McAb)识别。纯化的D-双聚体用ELISA检测,证实不含有E片段。纯化的E片段分子量约7.47×10~(-23)kg,还原后形成α链片段(分子量约1.03×10~(-23)kg)、β链片段(分子量6.14×10~(-24)kg)和γ链片段(分子量1.49×10~(-23)kg)3条肽链,可与抗E片段McAb进行免疫学反应。
Both D-dimer and E fragments are degradation products of cross-linked fibrin. According to their isoelectric point, the method of isoelectric focusing to purify D-dimer and E fragment was established. The molecular weight of the purified D-dimer is about 3.32 × 10 ~ (-22) kg, containing γ-γ dimer fragment (molecular weight 1.36 × 10 ~ (-22) kg) -23) kg) and α-chain fragment (molecular weight 2.49 × 10 -2 (-23) kg). Western Blot results show that it can be recognized by anti-D-dimer monoclonal antibody (McAb). Purified D-dimer was detected by ELISA and confirmed to contain no E fragment. The purified E fragment has a molecular weight of about 7.47 × 10 -2 (-23) kg, reduced to form an α chain fragment (molecular weight of about 1.03 × 10 -2 3 kg), a β chain fragment (molecular weight of 6.14 × 10 ~ (-24) kg ) And γ chain fragment (molecular weight 1.49 × 10 -23 kg), which can be immunologically reacted with anti-E-segment McAb.