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用DEAE-纤维素(DE-22)并改进酶的提纯方法,聚丙烯酰胺凝胶电泳为单一蛋白区带的酶制剂比活力为359μM/mg(E)/min(Npp为底物)·用凝胶过滤法测得该酶分子量为58600,氨基酸组成有20种,共467个氨基酸残基。10~(-4)M pCMB使酶活力丧失98％;1×10~(-3)M PMSP该酶活力丧失96％,2×10~(-4)M NBS以及1×10~(-2)M BrAc酶活力均丧失90％·酶修饰失活呈一级反应,修饰过的酶紫外280nm吸收峰消失·初步判断文昌鱼AcPase分子上的Cys,Trp,His,Ser等氨基酸残基可能是酶的活力必需基团。
Using DEAE-cellulose (DE-22) and improving the enzyme purification method, the specific enzyme activity of polyacrylamide gel electrophoresis as a single protein band was 359 μM / mg (E) / min (Npp is substrate) The molecular weight of this enzyme was 58,600 and the total number of amino acids was 20 with a total of 467 amino acid residues. 10 ~ (-4) M pCMB resulted in the loss of 98% of the enzyme activity; 1 × 10 -3 M PMSP lost 96% of the activity, 2 × 10 -4 M NBS and 1 × 10 -2 ) M BrAc enzyme activity was lost 90% · Enzyme modified inactivation was a first-order reaction, the modified enzyme UV 280nm absorption disappears · preliminary judgment Acanthopanax AcPase molecules Cys, Trp, His, Ser and other amino acid residues may be Enzyme vital groups.