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香蕉果实后熟过程中果皮乙烯释放先于果肉组织。果肉磷脂酶活性变化出现最早,呈一峰形升高过程,随后的顺序是细胞膜透性增大,乙烯释放及呼吸跃变。ACC促进及CCCP抑制香蕉果实乙烯的释放表明,其乙烯的形成是通过蛋氨酸途径。 应用CEPA、AOA及CCCP可改变细胞膜透性增大与乙烯释放的先后次序,CEPA使果实乙烯释放的时间提前到与膜透性增大几乎同时发生,经AOA与CCCP处理,细胞膜透性的增大推迟到乙烯高峰出现之后,看来这两者可能不存在因果关系。 1mM ACC可激活未发动后熟香蕉果实果皮及果肉组织乙烯生物合成的机制,CCCP对CEPA诱导释放乙烯的抑制作用表明,CEPA在体内主要通过酶解释放乙烯,CCCP对ACC及CEPA引起的乙烯释放有不同的抑制效应,推测这两者在体内有不同的酶解途径。CCCP未能抑制果皮诱导乙烯释放部分(约占总释放量的20％),可能是CEPA通过非酶途径产生的。
Banana fruit ripening in the process of peel ethylene release prior to the pulp tissue. The changes of phospholipase activity in flesh appeared earliest, showing a peak-shaped increase process, followed by the increase of cell membrane permeability, ethylene release and respiratory transition. ACC promotion and CCCP inhibition of banana fruit ethylene release showed that its ethylene formation is through the methionine pathway. Application of CEPA, AOA and CCCP can change the order of increase of cell membrane permeability and ethylene release. CEPA causes the release of ethylene in fruit to advance with the increase of membrane permeability almost at the same time. After AOA and CCCP treatment, the increase of cell membrane permeability Postponed to the peak of ethylene appears, it seems that there may be no causal relationship between the two. 1mM ACC could activate the biosynthesis of ethylene in pericarp and pulp tissue of unmanaged postharvest banana fruit. The inhibitory effect of CCCP on CEPA-induced ethylene release indicated that CEPA releases ethylene mainly through enzymolysis in the body, and CCCP has the effect on ACC and CEPA-induced ethylene release There are different inhibitory effects, suggesting that the two have different enzymatic digestion pathways in the body. CCCP failed to inhibit the peel-induced ethylene release part (about 20% of the total release), probably through the CEPA non-enzymatic pathway.