Based on gas chromatography and gas chromatography mass spectrometry techniques, the authors examined biomarker characteristics of the Ganquan Formation source rocks in the area of Dahulishan, Ejina, and analyzed the information and petroleum geological significance that were indicated by the source of organic matter, sedimentary environment and maturity and so on. Gas chromatography peak of saturated hydrocarbons from the Ganquan Formation source rocks showed “the former peak” of the single peak distribution, the main peak of carbon being C18 , indicating the main sources of organic matter with marine organic matter. Pr/Ph ranges from 0.3 to 0.6, indicating a strongly reducing sedimentary environment. Biomarker assemblages of the Ganquan Formation source rocks are abundant in tricyclic terpane with long side chains, with a high level of gammacerane, showing dominant distribution of C27 sterane. High contents of gammacerane in organic matter show organic facies of source rocks in the saltwater sedimentary environment. That average hopane/sterane ratio is 0.59, showing that algae have made more contributions to organic matter than bacteria. And the high level of C27 steranes shows that algae are the main source of hydrocarbon precursors. The Ganquan Formation’s methylphenanthrene index, which is an effective molecular parameter index to measure thermal evolution of organic matter, ranges from 0.35 to 0.50, and the conversion of vitrinite reflectance Rc from it is within the range of 2.00-2.09, indicating that the Ganquan Formation source rocks are in the over-mature stage of thermal evolution. Based on gas chromatography and gas chromatography mass spectrometry techniques, the authors examined biomarker characteristics of the Ganquan Formation source rocks in the area of ​​Dahulishan, Ejina, and analyzed the information and petroleum geological significance that were indicated by the source of organic matter, sedimentary environment and maturity and so on. Gas chromatography peak of saturated hydrocarbons from the Ganquan Formation source rocks showed “the former peak ” of the single peak distribution, the main peak of carbon being C18, indicating the main sources of organic matter with marine organic matter. Pr / Ph ranges from 0.3 to 0.6, indicating a strongly reducing sedimentary environment. Biomarker assemblages of the Ganquan Formation source rocks are abundant in tricyclic terpane with long side chains, with a high level of gammacerane, showing dominant distribution of C27 sterane. High contents of gammacerane in organic matter show organic facies of source rocks in the The average hopane / sterane ratio is 0.59, showing that algae have made more contributions to organic matter than bacteria. And the high level of C27 steranes shows that algae are the main source of hydrocarbon precursors. The Ganquan Formation’s methylphenanthrene index, which is an effective molecular parameter index to measure thermal evolution of organic matter, ranges from 0.35 to 0.50, and the conversion of vitrinite reflectance Rc from it is within the range of 2.00-2.09, indicating that the Ganquan Formation source rocks are in the over -mature stage of thermal evolution.